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John Thomas Woolhouse was born in Halstead, Essex, in 1666. Son of Thomas Woolhouse, royal oculist and of the third generation, according to Woolhouse, to have followed that profession. He was educated at Westminster School and matriculated in 1684 at Trinity College, Cambridge, on a scholarship. He graduated in 1686/7 and then travelled throughout Europe to familiarise himself with the various methods of treating diseases of the eye. He started a practice in London, and served for a time as Groom of the Chamber to King James II. He was working in Paris from before 1700 to about 1730. He served as surgeon to the Hospice des Quinze-Vingts in 1711. He originated the operation of iridectomy to restore sight in cases of occluded pupil, and he was the first to describe the complete and systematic extirpation of the lachrymeal sac when the duct was blocked. He was elected Fellow of the Royal Society of Surgeons of England, in 1721, being at that time oculist to the French King. He was also a member of the Royal Academy of Berlin and of the Institute of Sciences of Bologna. He died in 1734.