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Samuel Crompton was born in Berry Fold House, Over Darwen, Lancashire, in 1817. He was apprenticed to his uncle Samuel Barton, an opthalmic surgeon in Manchester (possibly 1790-1871; MRCS 1811 and FRCS 1844; surgeon to the Manchester Eye Hospital from 1815). He received his medical education partly at the Manchester School of Medicine, Pine Street, and St Bartholomew's Hospital, London, from 1838. He became a Member of the Royal College of Surgeons of England, and a Licentiate of the Apothecaries Society in 1839, and later MD St Andrews, in 1862. He returned to Manchester to practice in 1840. He became surgeon to Henshaw's Blind Asylum, Old Trafford, c 1849. He was Consultant Physician at Salford Royal Hospital and Dispensary. He published a treatise entitled Results of an Investigation into the Causes of Blindness, with Practical Suggestions for the Preservation of the Eyesight, in 1849. He retired to Cranleigh, Surrey, in 1881. He died in 1891.