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Whitlock Nicholl was born in Treddington, Worcester, in 1786. He grew up with his uncle, the Reverend John Nicholl. He was placed with Mr Bevan in 1802, a medical practitioner at Cowbridge in Glamorganshire. He entered as a pupil at St George's hospital, in 1806. He attended the lectures of Mr Wilson, Dr Hooper, Dr Pearson, Dr John Clarke, and Sir Everard Home. He was appointed house surgeon at the Lock Hospital, in 1808, and admitted as a member of the Royal College of Surgeons, in 1809. He returned to Cowbridge and entered into partnership with his former master, Mr Bevan, and then succeeded him as physician on his retirement. He was created Doctor of Medicine by Marischal College, Aberdeen, in 1816, and was admitted an extra Licentiate of the College of Physicians, the same year. He was created Doctor of Medicine by the Archbishop of Canterbury, in 1817, through the interest of his relation Sir John Nicholl. He had a successful practice in Ludlow. He matriculated from Glasgow in 1825, and attained the M D in 1826. He then moved to London, where he was admitted a Licentiate of the College of Physicians in 1836. He was elected a Fellow of the Royal Society, in 1830. He died in 1838.